The effect of auxin exposure on the adult Drosophila transcriptome

Auxin is used to induce transgene expression or degrade proteins in Drosophila. Here we exposed three different genotypes to 0 mM or 10 mM auxin and measured changes in gene expression for both males and females. We found that auxin exposure significantly alters the transcriptome of adult Drosophila in all genotypes tested. Overall design: To test the affects of auxin on the transcriptome, we fed 4-5 day old adult Drosophila to 0 mM or 10 mM auxin for 48 hours. We then processed whole body samples for RNA extraction and sequencing.