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Dengue Virus Replicative Intermediate RNA Detection by Reverse Transcription-PCR

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PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC119872/
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资源简介:
Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.
提供机构:
American Society for Microbiology (ASM)
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