Gene and retrotransposon expression analysis for WT, Pld6 KO, and Dnmt3l KO male germ cells during spermatogenesis

mRNA sequencing analysis of P0 testes and FACS-purified leptotene/zygotene (L/Z) spermatocytes. Compare transcriptomes of WT, Pld6 KO, and Dnmt3l KO germ cells to study the effects of these mutations affecting piRNA biogenesis and DNA methylation, respectively.本数据集针对P0龄睾丸组织及经荧光激活细胞分选（FACS, Fluorescence-Activated Cell Sorting）纯化的细线期/偶线期（L/Z）精母细胞开展mRNA测序分析。通过比较野生型（WT, Wild Type）、Pld6基因敲除（Pld6 KO）与Dnmt3l基因敲除（Dnmt3l KO）生殖细胞的转录组，分别探究这两种突变对piRNA（Piwi-interacting RNA）生物发生与DNA甲基化的影响。