Genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking. Genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking

To address the issue of retention of somatic cell memory in iPSCs we compared methylation profiles of genetically matched human iPSCs derived from fibroblasts and blood. We were using the all-in-one type footprint free SeVdp-iPS system, for generation of uniform iPSC lines. Our results show that iPSC lines derived from the same donor are highly similar to each other. Experimental design: methylation profiles were assayed by Reduced Representation Bisulfite Sequencing (RRBS) for fibroblast-derived iPSC (N=8) and blood-derived iPSC (N=10) as well as isogenic parental fibroblasts and peripheral blood mononuclear cells (N=4 different donors: T14; T42; T53; T55). H9 human embryonic stem cell lines was used as control.