Phosphoproteomics Reveals the Effect of Ethylene in Soybean Root under Flooding Stress.

Data from ProteomeXchange, PXD ID: PXD000898. File: 51 0 4.mgf. Published as part of J Proteome Res. 2014 Oct 24 . From the Abstract: {{i}} To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each time point. After 3 h of flooding exposure, the fresh weight of soybeans increased, whereas the ATP content of soybean root tips decreased. Using a gel-free proteomic technique, a total of 114 phosphoproteins were identified in the root tip samples, and 34 of the phosphoproteins were significantly changed with respect to phosphorylation status after 3 h of flooding stress ... {{/i}}