Effects of Y-27632 on Viability and c-kit+ Expression of CSCs.

(A) Cell viability assay using Calcein-AM. CSCs were treated with Y-27632 (0.0, 0.1, 1.0, & 10.0μM) for 48hrs followed by Calcein-AM staining. Representative images (a1 to a4) of fluorescent microscopy display the live cells (in green). Scale bar = 200μm. Relative cell viability (%) was analyzed by normalizing the means of fluorescent intensity to the control level as 100%, shown in the bar graphs on the right. *: p(B) The c-kit+ expression of CSC in Control and 48hrs after 10μM Y-27632 treatment. Adherent CSCs were stained with anti-human c-kit+ specific antibody followed by the secondary antibody-conjugated with FITC. C-kit+ CSCs are shown in green (b1, b3, b4, & b6) and nuclei are in blue (b2, b3, b5, & b6). Scale bar = 100μm. Percentages of c-kit+ CSCs from both Control and Y-37632-treated groups are shown in the bar graphs on the right. No significance was observed between the two groups (ns: no significance, n = 3).