Asymmetric GUVs: inner and outer leaflets with anti-registered phase separation

The data provided are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric giant unilamellar vesicles (aGUVs). We observed that a few percent of aGUVs (5-10%) prepared in our experiments show anti-alignment of domains between the inner and the outer leaflet (see Data in Brief report). This dataset shows fluorescence micrographs obtained using scanning fluorescence confocal microscopy. For the system chosen, DSPC/DOPC/POPC/chol, the fluorescence signal of (1-palmitoyl-2-(dipyrrometheneboron difluoride)undecanoyl-sn-glycero-3-phosphocholine) TopFluorPC (TFPC) and 1,1'-Dioctadecyl-3,3,3',3'- Tetramethylindodicarbocyanine Perchlorate (DiD) show anti-alignment of the brighter domains on aGUVs.
We also compare the modulated phases observed in GUVs and aGUVs. For these datasets, we collected a z-stack of micrographs. Images were collected in a scanning microscope Nikon Eclipse C2+ (Nikon Instruments, Melville, NY).