Transcriptomic analysis of Aspergillus flavus in response to the solvent Dimethyl Sulfoxide

Dimethyl sulfoxide (DSMO) is a simple molecule widely used because of its great solvating ability. Beyond its physico-chemical properties, it is also biologically active, including on fungal species. Aspergillus flavus is a saprophytic and famous pathogenic fungus able to produce Aflatoxin B1 (AFB1), a potent carcinogenic mycotoxin which may contaminate many food crops. The aim of this study was to characterize the effect of DMSO on A. flavus transcriptome profile using high-throughput RNA-sequencing assay. Six replicated fungal mycelium cultures were grown in three conditions (CTRL, DMSO Dose 1, DMSO Dose 2) to generate 18 samples in total which were extracted and processed to perform a RNAseq study.