Targeted metabolomics data for dimethyl-ribityl lumazine (DMRL) and riboflavin in Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway

This dataset contains raw mutliple reaction monitoring (MRM) data for
intermediate metabolites of the riboflavin biosynthetic pathway (RBP) in
wild-type and riboflavin biosynthetic pathway gene deletion mutants
of Mycobacterium
tuberculosis and Mycolicibacterium smegmatis. These
strains were generated to investigate the source of metabolic antigens
recognized by mucosal-associated invariant T (MAIT) cells, which detect
intermediates from the riboflavin biosynthetic pathway. To determine the
impact of these gene deletions on the production of riboflavin and its
precursors, we developed an MRM assay for DMRL and Riboflavin. This
dataset provides evidence of how these disruption impact the biosynthesis
of DMRL and Riboflavin which has enables a better understanding of the
source of MR1 antigen in mycobacteria.