Time-Series Dataset of Spectroscopy and Active Fluorescence Response to Nitrogen Deficit in Wheat

This dataset assessed the potential of sun-induced chlorophyll fluorescence to estimate plant net photosynthesis using a series of linear and non-linear light-use efficiency models (Cendrero-Mateo et al., 2025). In a wheat field exposed to different nitrogen treatments, coincident measurements of gas exchange, active fluorescence, reflectance, and passive fluorescence were made once a week on the same leaves under the same environmental conditions after two months of plant growth (i.e., beginning of tillering) for nine measurement days from February 24 to April 27. Measurements were always made under clear sky conditions during the morning hours (09:00 to 11:00). Each day, three fully expanded flag leaves exposed to direct sunlight were selected per replicate and treatment (number of leaves per treatment per day = 9, total number of leaves per day = 27). This dataset contains three different files: [1] complile_dataset: Excel file with the main parameters used in the Cendrero-Mateo et al. (2025) study. [2] fluowat: retrieved parameters derived from the fluowat leaf clip measurements (Alonso-Chordá, 2022) [3] pigment_effective_abs: retrieved parameters derived from the spectral fitting method described in Van Wittenberghe et al. (2022) and Van Wittenberghe et al. (2024). A full description of the experiment, along with the scientific results, is published by Cendrero-Mateo et al. (2025) and can be found at: 10.1109/JSTARS.2025.3562256. Please refer to the readme included in the Excel and zip files for more detailed information. References: Cendrero-Mateo M.P. et al., 2025. Use of Sun-Induced Chlorophyll Fluorescence in Linear and Non-Linear Light Use Efficiency Models for Remote Estimation of Plant Photosynthesis Under Stress Conditions. IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing. 10.1109/JSTARS.2025.3562256. Van Wittenberghe S. et al., 2024. Retrieval of leaf-level fluorescence quantum efficiency and NPQ-related xanthophyll absorption through spectral unmixing strategies for future VIS-NIR imaging spectroscopy. Remote Sensing of Environment. https://doi.org/10.1016/j.rse.2023.113879 Alonso Chordá, L. 2022. Passive direct measurement of sun-induced chlorophyll fluorescence spectrum from in vivo leaves [Doctoral thesis]. https://rodrigo.uv.es/handle/10550/82384 Van Wittenberghe S. et al., 2021. Combined dynamics of the 500–600 nm leaf absorption and chlorophyll fluorescence changes in vivo: Evidence for the multifunctional energy quenching role of xanthophylls. Biochimica et Biophysica Acta (BBA) - Bioenergetics. https://doi.org/10.1016/j.bbabio.2020.148351 Cendrero-Mateo M.P. et al., 2016. Plant chlorophyll fluorescence: active and passive measurements at canopy and leaf scales with different nitrogen treatments. J. Exp. Bot. https://doi.org/10.1093/jxb/erv456