Site-specific conjugation of maleimide benzophenone to Z domain variants.
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(A): Schematic showing how maleimide benzophenone is conjugated to a unique cysteine residue introduced into each of the Z variants by genetic engineering. (B): A representative result from analysis by LC-ESI mass spectrometry of the product obtained after conjugation (ZF5I-Q32C-MBP). (C): Schematic showing the 16 different Z-MBP probes (eight positions for the MBP group on both the ZWT and ZF5I variants) evaluated for photocoupling to Fc of mIgG1 using UV radiation.
创建时间:
2016-02-24



