1PN zygote

This study aimed to demonstrated the utilization value of 1PN embryos. The embryo development, the pronuclear characteristics, and the genetic constitutions were investigated. The blastocyst formation and good-quality blastocyst rates in 1PN zygotes were 22.94% and 16.24%, significantly lower than those of 2PN zygotes (63.25% and 50.23%, respectively, P=0.000). Compared to arrested embryos, 1PN zygotes that developed into blastocysts showed significantly larger area (752.57±131.53 vs. 653.65±116.58, P=0.000), longer diameter of pronuclear (30.10±2.90 vs. 27.28±2.83, P=0.000), and a greater number of nuclei (11.92± 4.06 vs. 7.72±3.07, P=0.000). Of the 86 tested embryos, the diploidy euploidy rate was significantly higher in good-quality blastocysts comparison with the arrested embryos (66.67% vs. 11.76%, P=0.000), which was also significantly higher in IVF-1PN blastocysts than in ICSI-1PN blastocysts (75.44% vs. 25.00%, P=0.001). However, the pronuclear characteristics were not related to the chromosomal ploidy once they formed good-quality blastocysts. In conclusion, the pronuclear characteristics in 1PN zygotes are related to their developmental potential. Those zygotes developed into blastocysts have unique pronuclear characteristics and significantly higher rate of diploidy euploidy in comparison with the arrested embryos. The 1PN blastocysts are recommed to be used after genetic testing, especially for those from IVF cycles.

本研究旨在阐明单原核（1PN）胚胎的应用价值。本研究对胚胎发育情况、原核特征及遗传构成展开了调查分析。单原核受精卵（1PN zygotes）的囊胚形成率与优质囊胚率分别为22.94%和16.24%，显著低于双原核受精卵（2PN zygotes）的63.25%与50.23%（P=0.000）。与停滞发育的胚胎相比，发育至囊胚阶段的1PN受精卵具有显著更大的面积（752.57±131.53 vs 653.65±116.58，P=0.000）、更长的原核直径（30.10±2.90 vs 27.28±2.83，P=0.000）以及更多的细胞核数量（11.92±4.06 vs 7.72±3.07，P=0.000）。在86枚受检胚胎中，优质囊胚的二倍体整倍体率显著高于停滞发育胚胎（66.67% vs 11.76%，P=0.000）；体外受精（IVF）来源的1PN囊胚（IVF-1PN blastocysts）的二倍体整倍体率也显著高于卵胞浆内单精子注射（ICSI）来源的1PN囊胚（ICSI-1PN blastocysts）（75.44% vs 25.00%，P=0.001）。不过，当1PN受精卵发育为优质囊胚后，其原核特征与染色体倍性并无关联。综上，1PN受精卵的原核特征与其发育潜能密切相关。相较于停滞发育的胚胎，发育至囊胚阶段的1PN受精卵具备独特的原核特征，且二倍体整倍体率显著更高。建议1PN囊胚在经过遗传学检测后再投入使用，尤其针对体外受精（IVF）周期来源的1PN囊胚。

搜集汇总

背景与挑战

背景概述

该数据集名为'1PN zygote'，发布于2023年，专注于研究单原核合子的胚胎发育潜力、原核特征与遗传组成。关键发现包括：1PN合子的囊胚形成率和优质囊胚率显著低于2PN合子，但发育成囊胚的1PN合子具有独特的原核特征和更高的二倍体整倍体率，尤其在IVF周期中。数据集为受限访问，数据量6.14 MB，包含1个文件，适用于生殖医学和胚胎学研究。