Experimental Assessment of Active Homeostasis Regulation for Extending Mammalian Lifespan by Prolonging the Youth Phase

Idea of the Work /1/: Hypothesis 1: Within a living organism, the fluids forming the basis of intercellular and intracellular fluids, plasma and lymph, are chemically H₂O, yet their physical properties differ fundamentally from those of drinking water. For example, physiological saline, which closely mimics the chemical composition of blood plasma, can only be intravenously administered in strictly limited amounts and at a controlled rate, due to its significant physical disparity from blood plasma. Moreover, within the body, the physical parameters of fluids are maintained in a non-equilibrium state, requiring constant effort from the organism to keep them within the so-called physiological norm, a phenomenon referred to as homeostasis in biology and medicine. Hypothesis 2: The physical characteristics of homeostasis serve as the foundation for its biochemical parameters. Therefore, maintaining these physical parameters actively within the physiological corridor substantially aids the organism in stabilizing its biochemical homeostasis and reduces the overall energy expenditure required to do so. Hypothesis 3: Active regulation of the physical parameters of homeostasis, ensuring they remain within physiological limits throughout all stages of life, including under adverse internal and external influences, allows the organism to conserve energy and biological resources during its lifespan. If these hypotheses hold true, then active homeostasis control enables the body’s cells to exist in a "gentle mode." This would allow the cells to complete their life cycles in the time naturally allotted to them. As a result, cell divisions in the experimental group occur less frequently compared to the control group. Given the biological limit on cell division (e.g., telomere shortening with each division), cells—and therefore organs—in the experimental group would not only live longer but also retain youthful characteristics for a significantly extended period.