RNA-Seq of hippocampus in Diversity Outbred mice

To understand the genetic regulation of gene expression and patterns of gene co-expression, we sequenced the transcriptome of the hippocampus of 258 Diversity Outbred (DO) mice of both sexes. DO mice (fourth and fifth generations of outcrossing) were sacrificed between 6-8 weeks of age and hippocampus dissected. Total hippocampal RNA was isolated using a TRIzol Plus RNA purification kit (Life Technologies) and mRNA sequencing library was prepared using a TruSeq kit (Illumina), both according to manufacturer's protocols. Paired-end 100bp reads were obtained using the Illumina HiSeq 2000.为解析基因表达的遗传调控机制与基因共表达模式，我们对258只兼具雌雄两性的多样性远交（Diversity Outbred, DO）小鼠的海马体转录组开展了测序。DO小鼠为远交繁殖的第4代与第5代个体，于6至8周龄时处死并剥离海马体。总海马体RNA采用TRIzol Plus RNA纯化试剂盒（Life Technologies）提取，mRNA测序文库则使用TruSeq试剂盒（Illumina）构建，所有操作均严格遵循厂商说明书流程。本研究采用Illumina HiSeq 2000平台获取了100bp双端测序读段。