Identification of L1TD1-associated transcripts in DNMT1 KO HAP1 cells

In this study, we aimed at determining RNA interactors of L1TD1 RNPs. To achieve this, DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cells were generated by Crispr/Cas9 gene editing tool and L1TD1 RIP was performed by using these cell lines. Our study reveals a comprehensive analysis of differentially enriched transcripts in L1TD1 containing RNPs. We performed RIP-seq by L1TD1 immunoprecipitation followed by RNA isolation from immunoprecipitated complexes using DNMT1 KO and DNMT1 L1TD1 DKO HAP1 cells in biological triplicates. Differential enrichment analysis was done using DESeq2. 10% input used as a reference to for the enrichment of the transcripts in relate to their expression. DNMT1 L1TD1 DKO cells were used as a negative control as they lack L1TD1 expression.