The Complex Subunit VdAP-2α Regulates Polar Growth, Ergosterol Metabolism, and Virulence in Verticillium dahliae

Figure 1. Identification and subcellular localization of VdAP-2α in Verticillium dahliae.Figure 2. VdAP-2α regulates vegetative growth and sporulation in Verticillium dahliae.Figure 3. VdAP-2α regulates microsclerotia development and melanin deposition in Verticillium dahliae.Figure 4. Comparative transcriptomic analysis of the functional regulation of VdAP-2α in Verticillium dahliae.Figure 5. VdAP-2α affects polar growth and development in Verticillium dahliaeFigure 6. VdAP-2α influences ergosterol biosynthesis in Verticillium dahliae.Figure 7. VdAP-2α affects the pathogenicity of Verticillium dahliae on in Acer truncatum.Figure S1. Analysis of the T-DNA insertion site in mutant 166.Figure S2. Analysis of polymerase chain reaction (PCR) for deletion and complementation of the VdAP-2α gene in Verticillium dahliae.Figure S3. Transcriptomic analysis of differentially expressed genes (DEGs).Figure S4. WT, ΔVdAP-2α, ECΔVdAP-2α strains' stress responses to voriconazole and terbinafine, and standard curves for squalene, lanosterol, and ergosterol quantification.Figure S5. Pathogenicity of the wild-type, VdAP-2α mutant, and complemented strain on cotton seedlings.Figure S6. Comparative transcriptome analysis of Verticillium dahliae related to ergosterol, polar growth, and melanin.Figure S7. VdAP-2α regulates melanin deposition in Verticillium dahliae.Figure S8. Fluorescence intensity analysis of CFW staining in hyphae of different strains