Viral recovery rate of collection and quantification system.

aThe experiments were performed in triplicate for the high amount experiment controls and the number is the average and standard error (SE) of the results.
bThe low dose experiment was performed once to confirm the reproducibility of the recovery rate, as determined by the high dose experiment.
cAverage copy number of the influenza A M1 gene in the gelatin membrane.
dPercentage of viral load on the gelatin membrane to that of collection when the nebulizer was set close to the membrane.
eThe nebulizer was set 10 cm from the gelatin membrane filter through which the viral mist was suctioned.
fThe cone-shaped, megaphone-like device of 50 cm in length was connected with the gelatin membrane filter and the nebulizer was set at the entrance of the device.
gPercentage of viral load in the product of each step to those at the start of each step.
hViral load in the viral fluid poured into the gelatin membrane solution.
iViral load in the solution of the gelatin membrane filter dissolved in 10 mL of MEM.
jThe gelatin solution containing the virus was digested with 10 µg/mL of collagenase.
kThe digested fluid was ultra-centrifuged at 125,000 g for 100 min and the viral RNA was extracted from the precipitate and concentrated by freeze-drying and vacuum centrifugation.