Transcriptome profile of mesenchyme tissues of the pharyngeal arches involved in thymus formation in chicken embryos

In this study we perform the transcriptomic analysis of embryonic tissues involved in thymus formation. Using the chick-quail model, we identified thymus-forming potential in both non-canonical second PP (2PP) and canonical third/fourth pp (3/4PP) endoderm. We further identified the 3/4PA and ventral region of 2PA (2PAv) mesenchyme as positively regulate this potential, while the dorsal region of 2PA (2PAd) exerts inhibitory effects. The transcriptomes of 2PAv, 2PAd and 3/4PA mesenchyme were obtain from isolated tissues of chicken embryos with 2.5 days of development.