Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance

A fundamental issue in the metabolic field is whether it is possible to understand underlying mechanisms that characterize individual variation. Whole-animal performance relies on mitochondrial function as it produces energy for cellular processes. However, individual variation in mitochondrial traits lack of longitudinal measures to evaluate how mitochondrial function can change within and among individuals and with environmental context. The aims of this study were to test the repeatability of muscle mitochondrial metabolism thanks to two biopsies performed in red muscle, and to evaluate effects of biopsies on whole-animal performance in goldfish Carassius auratus. Our results show that basal mitochondrial respiration and net phosphorylation efficiency are repeatable at 14-day intervals. We also show that swimming performance (optimal cost of transport and critical swimming speed) was repeatable in biopsied fish, whereas the repeatability of individual oxygen consumption (standard and..., , , # Data from: Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance

[https://doi.org/10.5061/dryad.2v6wwpzvn](https://doi.org/10.5061/dryad.2v6wwpzvn)

This file includes different documents related to the article \"Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance\", dealing with the validation of the muscle biopsy method, on 12 cm goldfish.

In particular:

\"ESM 1_Supplemental Data\" contains supplemental tables and figures to give more details about the experimental protocols as well as about preliminary measurements to validate the muscle biopsy method.

\"ESM2 Thoral et al Dataset\" contains all the data presented in the manuscript as well as the data presented in \"ESM 1Supplemental Data\".

* The sheet \"Whole animal performance traits\" contains data presented in Figure 3 and Table 1. The column \"Treatment\" contains the 2 groups : control fish and biopsied fis...