Genome wide host gene expression analysis in chicken lungs infected with avian influenza viruses. Gallus gallus

The mechanisms responsible for the molecular pathogenesis of the highly pathogenic avian influenza virus (HPAIV) or low pathogenic avian influenza virus (LPAIV) in avian species remain poorly understood. Thus, global immune response of chickens infected with HPAIV H5N1 (A/duck/India/02CA10/2011) and LPAIV H9N2 (A/duck/India/249800/2010) viruses was studied using microarray to identify crucial host genetic components responsive to these infection. HPAIV H5N1 induced excessive mRNA expression of cytokines (IFNA, OASL, MX1, RSAD2, IFITM5, GBP 1, IL1B, IL18, IL22, IL13, IL12B, CCL4, CCL9, CCL10, CX3CL1 etc) in lung tissues. This excessive cytokine response (cytokine storms) may cause tissue damage and high mortality in chickens. In contrast, the expression levels of most of the cytokines remained unchanged in the lungs of LPAIV H9N2 virus infected chickens. This study indicated the relationship between host cytokines response and their roles in pathogenesis in chickens infected with HPAIVs. Overall design: Agilent Custom Chicken Gene Expression 8X60k (AMADID: G4102A_059389) designed by Genotypic Technology Private Limited , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

搜集汇总

背景与挑战

背景概述

该数据集使用微阵列技术分析了鸡感染高致病性H5N1和低致病性H9N2禽流感病毒后肺组织的全基因组宿主基因表达，发现H5N1诱导了强烈的细胞因子风暴（如IFNA、IL1B等），与高死亡率相关，而H9N2未引起显著细胞因子变化，揭示了宿主免疫应答差异与病毒致病性的关联。