Single nucleus multiome (RNAseq and ATACseq) of male mouse pituitary

We report the single nucleus multiome (RNAseq+ATACseq) of a male mouse pituitary sample. This dataset was generated for supporting the development of a novel computational framework for analyzing gene regulatorty circuitry from single cell multiome datasets. This computational framework extract regulatory circuits consisting of TFs, cis-regulatory sites and target genes by modelling the co-incidence of the RNA levels of the TFs, the chomatin accessibility of the TFs' binding sites and the RNA levels of target genes across single cell. Specifically we identified gonadotrope-specific regulatory circuits under the transcription factor Gata2. These regulatory circuits were validated by male mouse pituitary samples with gonadotrope-conditional knockout of Gata2 previously reported in GSE190066. Single nucleus true multiome (RNAseq and ATACseq) of the pituitary tissue of a male mouse.本研究报道了雄性小鼠垂体样本的单细胞核多组学（single nucleus multiome，RNAseq+ATACseq）数据。本数据集的构建旨在支撑一款用于从单细胞多组学数据中解析基因调控环路的新型计算框架的开发。该计算框架通过建模单细胞层面中转录因子（transcription factors, TFs）的RNA表达水平、转录因子结合位点的染色质可及性，以及靶基因的RNA表达水平三者的共现关系，来提取由转录因子、顺式调控元件与靶基因构成的调控环路。具体而言，本研究鉴定出了以转录因子Gata2为核心的促性腺激素细胞特异性调控环路。上述调控环路已通过此前在GSE190066中报道的、携带有促性腺激素细胞特异性Gata2条件性敲除的雄性小鼠垂体样本得到了验证。本数据集具体为雄性小鼠垂体组织的单细胞核真实多组学（RNAseq与ATACseq）数据。