Transcriptional changes to cytotoxic stresses are driven by HSF-activated genes and enhancers [PRO-seq]

We utilized precicion run-on sequencing (PRO-seq) to analyze the roles of HSF1 and HSF2 in the reprogramming of transcription under oxidative stress and heat shock. PRO-seq was performed in wild-type (WT), HSF1 knock-out (HSF1 KO) and HSF2 knock-out (HSF2 KO) mouse embryonic fibroblasts (MEFs) that were treated with heat shock (HS) or oxidative stress induced by menadione (MD). Overall design: 2 replicates of PRO-seq was performed in WT, HSF1 KO and HSF2 KO MEFs that were exposed to HS (42 °C, 1 h) or MD (30 µM, 2 h). Please note that each processed data file was generated from both replicates and is linked to the corresponding Rep1 sample records.