Early activation of cytokines and transcription factors regulated by peripheral blood mononuclear cells (PBMC's) plays a role in black Bengal goats' resistance to Haemonchus contortus infection

Haemonchus contortus is one of the most economically important gastrointestinal parasites of small ruminants worldwide, particularly in tropical and subtropical regions. Its high pathogenicity, blood-feeding behavior, and increasing resistance to anthelmintic drugs pose a serious threat to livestock health and productivity. Understanding host immune responses that confer natural resistance to H. contortus is therefore critical for developing sustainable control strategies. The Black Bengal goat (BBG), a native Bangladeshi breed, shows resistance to H. contortus, with genetic polymorphisms linked to lower fecal egg counts and parasite expulsion. However, the immune mechanisms behind this resistance remain unclear. Therefore, this study investigates how PBMCs respond in naïve and primed BBG kids during the first 7 days of H. contortus infection using both in vivo and in vitro approaches.  This dataset contains immunological and parasitological data generated from naïve and primed Black Bengal goat kids experimentally infected with Haemonchus contortus. It includes quantitative gene expression data (via RT-qPCR) of key cytokines and transcription factors (e.g., IL-4, IL-5, IL-13, IL-33, MCP1, CXCL1, TLR2, GAL14), histological data on cellular infiltration (eosinophils, neutrophils) in the abomasal mucosa and lymph nodes from Day 0 to Day 7 post-infection, and parasitological counts of L4 larvae recovered from abomasum. The dataset also contains in vitro larval motility measurements of H. contortus L3 following co-culture with PBMCs from both naïve and primed kids, as well as fecal egg count data following adoptive transfer of primed PBMCs. The data are organized into multiple files by modality and timepoint, enabling reuse for studies on host–parasite immune responses, Th2 polarization, immunogenetics, and resistance biomarker identification.