Modeling Braf-induced thyroid cancer development and cell redifferentiation using pluripotent stem cell-derived organoids

To generate a papillary thyroid carcinoma organoid model by inducing the BrafV637E mutation in mouse embryonic stem cells derived functional thyroid follicles to better comprehend the oncogenic events driven by Braf-oncogene and develop a drug screening tool. We performed gene expression profiling analysis using data obtained from bulk RNA-seq of organoid model with and without induction of BrafV637E expression induction, after MEK and PI3K inhibitors addition). Comparative gene expression profiling analysis of bulk RNA-seq data of organoids developped from G4 RosaLuc TRE-Nkx2-1-Pax8_bTg-NES-BrafV637E-ERT2 mouse embryonic stem cells with addition of Br-cAMP, Br-cAMP+4-Hydroxytamoxifen and Br-cAMP+4-Hydroxytamoxifen+PD0325901+LY204002.