Pitaya transcriptome assemblies and investigation of transcript abundances II

The RNA-Seq dataset PRJNA627818 was re-analysed to check findings reported in the corresponding publication. RNA-Seq reads were trimmed with Trimmomatic (Bolger et al., 2014) and assembled with Trinity (Grabher et al., 2011). Encoded peptide sequences were predicted and functionally annotated using a previously developed approach (Haak et al., 2018). kallisto (Bray et al., 2016) was applied to estimate the abundance of transcripts based on transcriptome assemblies of three pitaya species and the raw RNA-Seq reads. Please see our corresponding manuscript for details.

对RNA-Seq数据集PRJNA627818进行了重新分析，以验证相关出版物中报告的发现。利用Trimmomatic（Bolger等，2014年）对RNA-Seq读数进行了剪切，并使用Trinity（Grabher等，2011年）进行组装。通过先前开发的方法（Haak等，2018年）预测编码肽序列并对其进行了功能注释。采用kallisto（Bray等，2016年）对基于三个龙眼物种转录组组装和原始RNA-Seq读数的转录本丰度进行了估算。具体细节请参阅我们相应的手稿。