Effect of Botrytis cinerea infection in Col-0 and erfVII pentuple mutant

Pathogens infection exerts major effects on gene expression, which is reconfigured to raise the defences of the attacked plant. In the recent years the establishment of an hypoxic environment during Botrytis infection was described. We used the pentuple erfVII mutant to evaluate the contribution of ERFVII members to plant reaction to Botrytis and their effect on gene expression. Each group is represented by one biological replicates. Each one was obtained by pooling 5 plants. Genotypes of Arabidopsis thaliana used included Col-0 ecotype (wild-type) and the pentuple erfVII mutant. Plants were grown in pots for 3-4 weeks at 23°C with a 12/12 h photoperiod at 120 μmol photons m–2 s–1 before being utilized for experiments. Plants were infected with Botrytis for 48h at high umidity before sampling.