Effect of fumarate analogs on human m-NAD(P)-ME and c-NADP-ME1,2.

1These values were the ratios of specific activities of the enzyme determined in the presence and absence of these chemical compounds.2These values were the average with standard deviation of three-time repeats.3The enzyme specific activities of the m-NADP-(P)-ME WT, R67A/R91A, K57S/E59N/K73E/D102S and c-NADP-ME WT were approximately 0.1, 0.005, 0.02 and 0.08 µmol/min, respectively, and the final enzyme concentration in an individual assay was 20, 400, 100, and 25 ng/µl, respectively.4For m-NAD(P)-ME, the final concentrations of these analogs were fixed at 5 mM, except for monoethyl fumarate and mesaconate, which were fixed at 10 mM and 20 mM, respectively.5For c-NADP-ME, the final concentrations of these analogs were fixed at 2 mM.