Zea mays KCS enzyme family analysis

Characterization of the plant fatty acid elongase (FAE) enzyme system that catalyzes the biosynthesis of very long chain fatty acids has been difficult because of large genetic and biochemical redundancy. In this study, we have heterologously expressed the 26 ZmKCS proteins in four different Saccharomyces cerevisiae strains to gain insight into the function of these putative isozymes. Collectively, this represents 83 experimentally modified yeast strains, plus five control strains.<br> <br> Data included within this entry are:<br> <br> 1) Growth of each strain measured as optical density (OD) 600 nm taken at different times in the growth of each strain. .<br> 2) The concentration of different fatty acids that each strain produced. Fatty acids were extracted from lyophilized yeast cell pellets, and methyl esters of fatty acids were analyzed by GC-MS and/or GC-FID; quantification was relative to an internal standard (19 carbon fatty acid) that was spiked into each sample. <br> 3) Transmission electron micrographs of the five yeast strains that are expressing ZmKCS proteins, which complement the lethality of the scelo2; scelo3 double mutant. <br> 4) Cell wall thickness measurements of the five yeast strains that are expressing ZmKCS proteins, which complement the lethality of the scelo2; scelo3 double mutant. 5) Images of the five yeast strains that are expressing <em>Zm</em>KCS, which complement the lethality of the <em>scelo2; scelo3</em> double mutant, grown on solid media.