Modeling the effect of prolonged ethanol exposure on global gene expression and chromatin accessibility in normal 3D colon organoids

In this study, we aimed to explore the biological effect of ethanol exposure on a human colon 3D organoid model. This model serves as a representation of healthy colon epithelial cells. In this small pilot study, RNA sequencing and ATAC sequencing data was generated on 3 healthy male pairs (treated and untreated) 72 hours post exposure. We identified a number of differentially accessible regions and differentially expressed genes relevant to colorectal cancer, which may help elucidate molecular mechanisms through which ethanol exposure increases disease risk.