Cell-free transcriptome of Barrett's Esophagus and Esophageal Cancer [Illumina - short read]

Esophageal adenocarcinoma (EAD) benefits from a large improvement in five-year survival when detected in early stages, therefore, early detection strategies are paramount to mitigating disease. Those diagnosed with Barrett's Esophagus (BE) are at higher risk for EAD, though there exists no routine screening for EAD. Recent advances in cell-free RNA sequencing aim to create minimally-invasive diagnostic modalities. We present short and long read data from plasma of BE, EAD, and Healthy patients for biomarker discovery. Overall design: CfRNA samples were sequenced using both long and short reads. Long reads were used for transcript discovery, appended to repeat element and GENCODE transcript annotation files to create an extended transcriptome