Transcription profiling of mouse liver of the KRAP deficient animals

KRAP (Ki-ras-induced actin-interacting protein) is a cytoskeleton-associated protein and a ubiquitous protein among tissues, originally identified as a cancer-related molecule. KRAP-deficient (KRAP-/-) mice show enhanced metabolic rate, decreased adiposity, improved glucose tolerance, hypoinsulinemia and hypoleptinemia. KRAP-/- mice are also protected against high-fat diet-induced obesity and insulin resistance despite of hyperphagia. Experiment Overall Design: Total RNA was extracted from livers of three pairs of littermates (KO1 vs. WT1, KO2 vs. WT2 and KO3 vs. WT3) fed normal chow.KRAP（Ki-ras诱导肌动蛋白互作蛋白，Ki-ras-induced actin-interacting protein）属于细胞骨架相关蛋白，在机体各组织中广泛表达，最初被鉴定为癌症相关分子。KRAP缺陷型（KRAP-/-）小鼠呈现代谢率升高、体脂降低、葡萄糖耐量改善、低胰岛素血症与低瘦素血症表型。尽管存在摄食过多的情况，KRAP-/-小鼠仍可抵御高脂饮食诱导的肥胖与胰岛素抵抗。实验整体设计：从3对同窝小鼠（KO1 vs WT1、KO2 vs WT2、KO3 vs WT3）的肝脏组织中提取总RNA，所有受试小鼠均饲喂普通饲料。