CD4+ T cells' immune response during the early phase of infection with Schistosoma japonicum

Single-cell transcriptome analysis reveals that the CD4+ T cells' immune response was more intense in the non-permissive host Microtus fortis than in the permissive host Kunming mice during the early phase of infection with Schistosoma japonicum We have employed a single-cell sequencing approach using Singleron scRNA-seq to explore the immune response of M. fortis and Kunming mice to S. japonicum. The study allowed us to decipher M. fortis PBMC heterogeneity and provide detailed information on the transcriptome profile of the different cell types following exposure to S. japonicum. Overall design: The peripheral blood mononuclear cells were isolated from uninfected and infected animals by density gradient centrifugation and analysed by scRNA-seq.