Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12171, Gallus gallus gallus, memory B cell. CIL. Dataset

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. INCENP localizes to centromeres in prometaphase, but fails to transfer to the spindle at anaphase in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for INCENP (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6D panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系细胞在分裂期的细胞核。采用Deltavision系统，通过100x/1.4NA计划阿波 objectives 镜头拍摄到的宽场免疫荧光图像的Z序列。图像经过约束迭代算法（Applied Precision）的重建处理。展示的是中心Z切片。INCENP在前期定位至着丝粒，但在野生型克隆18细胞中，在Aurora B抑制剂ZM447439的作用下，无法在后期转移到纺锤体上。细胞经INCENP（绿色）、α-微管蛋白（红色）和DAPI（蓝色）染色。详见Xu等人在2009年《细胞生物学杂志》第187卷第637-653页的图6D第1部分。