Global histone H3 lysine 9 trimethylation (H3K9me3) landscape changes in response to TGF-ß1

TGF-ß plays an important part in the Epithelial to Mesenchymal Transition (EMT) in many malignancies. TGF-ß may have pro- and anti-tumour effects in cancer cells, depending on the context and microenvironment. On the other hand, epigenetic alterations are becoming increasingly significant in a variety of biological activities. Aberrations in epigenetic processes can cause gene function to be disrupted, as well as malignant cellular change. Understanding the role of H3K9me3 in cancer survival is becoming increasingly popular. A worldwide drop in the heterochromatin mark H3 Lys9 dimethylation (H3K9me2), an increase in the euchromatin mark H3 Lys4 trimethylation (H3K9me3), and an increase in the transcriptional mark H3 Lys36 trimethylation were discovered during TGF-ß induced EMT (H3K36me3). Our study focuses on locating globally H3K9me3 related regions in response to TGF-ß since TGF-ß and H3K9me3 play an essential role in carcinogenesis. To further understand the role and process of H3K9me3 alteration in TGF-ß induced EMT, ChIP-Sequencing was done in PC-3 prostate cancer cells. Overall design: We performed Chromatin-Immunoprecipitation followed by Next-Generation Sequencing (ChIP-Seq) in PC-3 cells stimulated with TGF-ß1 for different duration. We used anti-H3K9me3 antibody to immunoprecipitate DNA fragment associated with H3K9me3. We found that a number of regions associated with H3K9me3 decreased in a time-dependent manner upon TGF-ß1 stimulation. Further the regions were classified into different Functional annotations.