Labelling of RC complexes in human cells by fusion to fluorescent proteins.

A. Mitochondrial RC complexes were labelled by fusion of single subunits to monomeric fluorescent proteins mEGFP, mDsRed and mDendra2, resp. at the indicated sites. B.C. Expression of the complex II subunit SDHB fused to GFP (CII-G) and its mitochondrial localization in stably transfected HeLa cells as visualized by fluorescence microscopy. D. Expression of the complex III subunit 10 (6.4 kDa UQCR) fused to GFP (CIII-G) and its mitochondrial localization in stably transfected HeLa cells as visualized by fluorescence microscopy. E. Expression of complex IV subunit 8a fused to hAGT and labelled with BG-TMR in stably transfected HeLa cells (CIV-hAGT-TMR). F. Expression of complex IV subunit 8a fused to GFP in stably transfected HeLa cells (CIV-G). G. Mitochondrial localization of complex V labelled at its γ-subunit by fusion to DsRed in stably transfected HeLa cells (CV-R). Scale bars: 20 µm (D, F, G); 5 µm (B, C), and 10 µm (E).