Examining Pathogenesis in Genome Instability Associated Neurodegenerative Mouse Model [TotalStranded-RNA-seq]

To investigate the molecular basis for ataxia in the DNA repair deficient mice we utilized RNA-seq to evaluate the transcriptome of AtmNes-cre;Aptx-/- (and AtmNes-cre;Parp1-/-) cerebellar and cortical tissue. Using total RNA from symptomatic 10-month-old double-mutant mice and littermate controls, we performed the transcriptome analysis. top 1,000 expressed genes in each genotype Total Stranded RNA sequencing of the cerebellum and cortex examining gene expression in ATM APTX null and associated control brains.