Single cell ATAC-seq analysis of human dermal fibroblasts and human pluripotent stem cells

Human pluripotent stem cells have two major pluripotent states, primed and naive, and the heterogeneity among cell lines in each pluripotent state remains a major unresolved problem. We showed that the overexpression of H1FOO-DD, which has a short expression period by fusing the destabilized domain to the maternal-specific linker histone H1FOO, together with OCT4, SOX2, KLF4 and LMYC in human somatic cells improves the quality of reprogramming to primed and naive pluripotency. Cultured human dermal fibroblasts and primed human pluripotent stem cell lines were sampled at specific time points, and analyzed by single cell ATAC-seq