Data from: Muscle diffraction at the life science x-ray scattering beamline

We report on recent methodological advances at the Life Science X-ray
Scattering (LiX) beamline of the National Synchrotron Light Source II
(NSLS-II) to support small-angle X-ray scattering experiments on skeletal
and cardiac muscle tissues. These experiments have been routinely
performed at the BioCAT beamline of the Advanced Photon Source (APS) over
the past two decades to measure sarcomeric protein organization within
healthy and diseased muscle tissues and provide direct molecular evidence
for their functional roles and dynamics. Many recent advances in our
understanding of sarcomeric proteins relied on diffraction data and
include, as examples, MyBP-C, crossbridge SRX/DRX states, and titin. With
LiX now available for muscle experimentation, more muscle users can be
supported which will speed up research of sarcomeric proteins, muscle
biomechanics, and skeletal and cardiac myopathies. LiX explicitly focuses
on high-throughput muscle diffraction with rapid sample turnover and
semi-automated data processing. These operations have been tested and
validated on skeletal and cardiac tissues sourced from both humans and
multiple animal models including pig, rat, mouse, and zebrafish.