Adult male fly hemolymph Lipidomics Data on Normal (ND) and High Sugar (HSD) for 14 days.

Frozen hemolymph samples from ND and HSD-fed flies were sent to the Northwest Metabolomics Research Center (NW-MRC) for targeted quantitative lipid profiling using the Sciex 5500 Lipidyzer as per methods established by NW-MRC120. The materials used include LC-MS grade methanol, dichloromethane, and ammonium acetate, all sourced from Fisher Scientific (Pittsburgh, PA). HPLC grade 1-propanol was obtained from Sigma-Aldrich (Saint Louis, MO). Milli-Q water was produced using an in-house Ultrapure Water System by EMD Millipore (Billerica, MA). The Lipidyzer isotope-labeled internal standards mixture, which contained 54 isotopes from 13 different lipid classes, was acquired from Sciex (Framingham, MA). HCER and LCER were not detected in Drosophila hemolymph, so we report 11 different lipid classes.