m6A-seq in glioblastoma stem cells. Homo sapiens

m6A RNA methylation plays an important role in regulating the self-renewal and tumorigenesis of glioblastoma stem cells. We performed m6A-seq analysis in glioblastoma stem cells with m6A levels modulated by knocking down METTL3 or METTL14 and identified m6A regulated genes in glioblastoma stem cells. Overall design: Glioblastoma stem cells were treated with lentivurs expressing constrol shRNA or target (METTL3 or METTL14) shRNA to knockdown the expression of METTL3 or METTL14. RNAs were extracted and m6A-seq analysis was performed using m6A specific antibody immunoprecipitation followed by RNA-seq.