PanCancer Immune Nanostring analysis of PBMCs stimulated with Innate-like T-cell agonists

We aimed to study the function of NKT, MAIT and V[gamma]9V[delta]2 T-cells in a population of people with cancer. To select appropraite immune parameters to study, we wanted to compare and contrast the expression of immune-related genes in human PBMCs following NKT, MAIT and V[gamma]9V[delta]2 T-cell activation. Aim: to compare the expression of immune-related genes in human PBMCs following NKT-cell activation with alpha-galactosylceramide, MAIT-cell activation with 5-amino-6-d-ribitylaminouracil/Methyl glyoxal, and V[gamma]9V[delta]2 T-cell activation with Bromohydrin pyrophosphate (BrHPP). PBMCs from six healthy donors in singleton were cultured with alpha-galactosylceramide (agc), Bromohydrin pyrophosphate (BrHPP), 5-amino-6-d-ribitylaminouracil/Methyl glyoxal (5aru) or media control for 72 hours. RNA was extracted using a commercially available kit and frozen before application to the PanCancer Immune nCounter Assay (NanoString Technologies, Inc.).