Supporting trees and alignments for the publication: Cryptic and abundant marine viruses at the evolutionary origins of Earth’s RNA virome

Whereas DNA viruses are known to be abundant, diverse, and commonly key
ecosystem players, RNA viruses are relatively understudied outside disease
settings. Here, we analyzed ≈28 terabases of Global Ocean RNA sequences to
expand Earth’s RNA virus catalogues and their taxonomy, investigate their
evolutionary origins, and assess their marine biogeography from pole to
pole. Using new approaches to optimize discovery and classification, we
identified RNA viruses that necessitate substantive revisions of taxonomy
(doubling phyla and adding >50% new classes) and evolutionary
understanding. “Species”-rank abundance determination revealed that
viruses of new phyla “Taraviricota”, a missing link in early RNA virus
evolution, and “Arctiviricota” are widespread and dominant in the oceans.
These efforts provide foundational knowledge critical to integrating RNA
viruses into ecological and epidemiological models.