Contrast agent calibration phantoms SPring-8 BL20B2

Contrast-enhanced imaging at SPring-8 BL20B2 beamline This dataset features a synchrotron radiation-based micro computed tomography dataset of different dilutions of ExiTron nano 12000 blood pool contrast agent. Data was recorded at the beamline BL20B2 of the synchrotron SPring-8 in May 2023. For more information, see the associated publication, repository or the FABRIC4 portal. The percentage of contrast agent is with respect to a concentration of 480 mg Ba/ml. Images in this repository were cropped from 2048 × 2048 × 1500 to the center 1024 × 1024 × 1500 voxels, in order to stay within Zenodo's 50 GB limit. Calib03_000perc_000mgBaml_absorption.zip0 % contrast agent, 0 mg Ba/ml Calib03_010perc_048mgBaml_absorption.zip10 % contrast agent, 48 mg Ba/ml Calib03_025perc_120mgBaml_absorption.zip25 % contrast agent, 120 mg Ba/ml Calib03_033perc_160mgBaml_absorption.zip33 % contrast agent, 158.4 mg Ba/ml Calib03_050perc_240mgBaml_absorption.zip50 % contrast agent, 240 mg Ba/ml These datasets were used to determine the contrast agent concentrations of contrast agent injected in vivo into the lateral ventricle of mice. The related dataset is: JP28, Intra-cerebroventricular infusion, time serieshttps://doi.org/10.5281/zenodo.15671325 Methods To correlate contrast agent concentrations with the linear attenuation coefficients, calibration scans were performed using different concentrations of the contrast agent. These solutions were prepared by diluting a customized 480 mg Ba/ml (1.5× concentrated compared to stock product, 320 mg Ba/ml) suspension of ExiTron nano 12000 barium nanoparticle-based contrast agent (Viscover nanoPET Pharma GmbH, Berlin, Germany) with isotonic mannitol solution (54.65 mg/ml, the same suspension medium as used by the manufacturer) to achieve final concentrations of 0, 48, 120, 158.4 and 240 mg Ba/ml. At each concentration, 4 µl of solution were drawn by a syringe pump into a 0.69 mm inner diameter PVC tube. These concentration samples were then imaged at a photon energy of 40 keV, provided by the beamline BL20B2’s double multilayer monochromator with 4.8% energy bandwidth. 1800 radiographs over a rotation range of 180° were acquired with a Hamamatsu Orca Flash4.0 v2 camera coupled with a tandem lens system (105 mm f/2.4 and 85 mm f/1.4) and a 500 µm LuAg:Ce scintillator for 8.0 µm effective pixel size. The field of view was reduced to 2048 × 1500 pixels due to the limited height of the X-ray beam, and images were recorded with 5 ms exposure time and 8 ms overhead time. Acquisition time per scan was 23 s. Sample-detector distance was 1 m.