Characterization of Naïve and Vitamin C‑Treated Mouse Schwann Cell Line MSC80: Induction of the Antioxidative Thioredoxin Related Transmembrane Protein 1

Schwann cells (SCs) are essential
in the production of the axon-wrapping
myelin sheath and provide trophic function and repair mechanisms in
the peripheral nerves. Consequently, well-characterized SC in vitro
models are needed to perform preclinical studies including the investigation
of the complex biochemical adaptations occurring in the peripheral
nervous system (PNS) under different (patho)­physiological conditions.
MSC80 cells represent a murine SC line used as an in vitro system
for neuropathological studies. Here, we introduce the most abundant
9532 proteins identified via mass spectrometry-based protein analytics,
and thus provide the most comprehensive SC protein catalogue published
thus far. We cover proteins causative for inherited neuropathies and
demonstrate that in addition to cytoplasmic, nuclear and mitochondrial
proteins and others belonging to the protein processing machinery
are very well covered. Moreover, we address the suitability of MSC80
to examine the molecular effect of a drug-treatment by analyzing the
proteomic signature of Vitamin C-treated cells. Proteomic findings,
immunocytochemistry, immunoblotting and functional experiments support
the concept of a beneficial role of Vitamin C on oxidative stress
and identified TMX1 as an oxidative stress protective factor, which
might represent a promising avenue for therapeutic intervention of
PNS-disorders with oxidative stress burden such as diabetic neuropathy.