A kinome-wide CRISPR screen identifies CK1a as a novel target to overcome enzalutamide resistance of prostate cancer

Utilizing a kinome-wide CRISPR-Cas9 knockout screen, we identified casein kinase 1 alpha (CK1a) as a novel therapeutic target to overcome enzalutamide resistance. To dissect the underlying mechanisms of targeting CK1a to sensitize cancer cells to ENZA, we performed RNA sequencing of 22Rv1 cells upon CK1a knockout or overexpression. We found that CK1a regulated double-strand break (DSB)-response signaling. Overall design: 1, CRISPR screen identifies novel targets to overcome enzalutamide resistance. 2, RNA sequening to address the mechanisms of targeiting the target to overcome enzalutamide resistance.