Mucosal gene expression profiling in ileum of patients with Crohn's disease

In this study, we investigated gene expression profiles in ileal mucosa from CD patients in different settings (post-operative CD (Rutgeerts score i0); post-operative recurrent CD (i1), post-operative recurrent CD (≥i2b), newly diagnosed CD and late stage CD)) and controls. Ileal mucosal biopsies were obtained during endoscopy from 65 CD (3 i0 CD, 6 post-operative recurrent (POR) i1 CD, 24 POR (≥i2b), 18 new and 14 late) patients and 12 normal controls. Total RNA was isolated from ileal mucosal biopsies, labelled and hybridized to Affymetrix Genechip Human Gene 1.0ST Arrays.