Transcriptome analysis of Control and FBXO38 knockdown B16-F10 tumor issue

F-box only protein 38 (FBXO38), which belongs to SKP1-CUL1-F-box protein (SCF) family, exhibits the ability to mediate Lys48-linked poly-ubiquitination and subsequent proteasome degradations of PD-1.To investigate the effect of FBXO38 knockdown on transcription in tumor, we performed RNA-seq in control and FBXO38 knockdown B16-F10 tumor issue. Overall design: We constructed control and FBXO38 knockdown by shRNA in B16-F10 cell lines. Tumor cells were then injected subcutaneously into C57BL/6 mice. After 16 days of culture, the mice were sacrificed by cervical dislocation, tumors were isolated, and RNA-Seq sequencing was performed on the tumor tissue.