Influence of the order of addition of fusing partners on HCVpp 1a fusion inhibition by Arb.

aArb (11.3 µM) was pre-incubated sequentially with either HCVpp or R18-labeled liposomes or both for the indicated times at 37°C before initiating lipid mixing by decreasing pH to 5.0 as described in the legend to Figure 2. The extent of inhibition of fusion by Arb was calculated relatively to the fusion observed in the absence of Arb and normalized to 100%. Results are the mean ± SEM of 3 separate experiments.