Supplementary data from: Single-cell analysis identifies ATC-like cells driving progression in relapsed follicular thyroid carcinoma

Follicular thyroid carcinoma (FTC) is prone to early distant metastasis and has a poor prognosis compared with papillary thyroid carcinoma (PTC). This study aimed to unravel the cellular and molecular mechanisms underlying FTC progression and its transformation into the aggressive anaplastic thyroid carcinoma (ATC). Through single-cell RNA sequencing (scRNA-seq) profiling of 46,739 cells from PTC, follicular variant PTC (FVPTC), relapsed FTC (RFTC), and ATC, we reconstructed a comprehensive molecular trajectory of thyroid carcinoma progression. Our analysis revealed that PTC, FVPTC, and FTC possess distinct yet converging pathways of dedifferentiating into ATC, with FVPTC also progressing to FTC. In RFTC, we identified a unique cluster of cells exhibiting ATC molecular characteristics. These cells interact with endothelial cells and fibroblasts mainly via the COL9A3-integrin α1β1 complex, and may exhibit high metabolic and proliferative potential. UBE2C was identified as a specific mark..., , # Supplementary data from: Single-cell analysis identifies ATC-like cells driving progression in relapsed follicular thyroid carcinoma

Dataset DOI: [10.5061/dryad.xksn02vw3](https://doi.org/10.5061/dryad.xksn02vw3)

## Description of the data and file structure

Supplementary Figures and Tables for \"Single-cell analysis identifies ATC-like cells driving progression in relapsed follicular thyroid carcinoma\"

### Files and variables

#### File: Supplementary_Materials-en.2025-00375.docx

**Description:** This file includes 4 figures and 3 tables. 

**Figure S1.** Flow diagram showing the design of this study.

**Figure S2.** To validate the overexpression and knockdown efficiency of UBE2C in FTC cells.

**Figure S3.** Analysis on the quality of metabolomics data.

**Figure S4.** Analysis on the metabolism regulated by UBE2C in FTC.

**Table S1.** The average expression of markers for each cell cluster

**Table S2.** The sequences of PCR primers used in this study

**Table S3.** The seque...,