Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence. Salmonella enterica subsp. enterica serovar Typhimurium strain:LT2

Salmonella encounters but survives host inflammatory response. To defend host-generated oxidants, Salmonella encodes primary antioxidants and protein repair enzymes. By repairing Met-SO to Met, Msrs maintain overall ROS homeostasis in cells and prevent ROS-mediated macromolecular damage. Earlier studies assessed the effect of deletions of one or two msrs on the stress physiology of S. Typhimurium. We generated a pan msr gene deletion strain in S. Typhimurium. Deletions of all msrs render S. Typhimurium highly prone to oxidative stress and attenuate its virulence.